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OBSERVE: After the coupling of the primary C-terminal amino acid of all peptides to the amino group of the membrane, the amino groups between the spots and likewise some of the amino teams throughout the spot areas don't type bonds with the amino acids. Ensure that all of the purification buffers are at 4 °C and perform the protein purification within the chilly room to avoid protein denaturation. WORD: This avoids the coupling of amino acids in the additional cycles with the membrane as an alternative of the growing peptide chain.
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Common posttranslational modifications embody methylation of the ε-amino group, giving methyl- , dimethyl- , and trimethyllysine The latter happens in calmodulin Other posttranslational modifications at lysine residues embrace acetylation , sumoylation , and ubiquitination Collagen comprises hydroxylysine , which is derived from lysine by lysyl hydroxylase - Glycosylation of hydroxylysine residues in the endoplasmic reticulum or Golgi apparatus is used to mark sure proteins for secretion from the cell In opsins like rhodopsin and the visible opsins (encoded by the genes OPN1SW , OPN1MW , and OPN1LW ), retinaldehyde forms a Schiff base with a conserved lysine residue, and interaction of light with the retinylidene group causes signal transduction in colour imaginative and prescient (See visible cycle for particulars).
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